Factors affecting oxidative phosphorylation by subcellular particles isolated from cotton seedling hypocotyls.

Properly prepared mitochondrial fractions from plant sources are capable of incorporating inorganic phosphate into high energy bonds upon the oxidation of members of the tricarboxylic acid cycle (3, 6, 7). Oxidative phosphorylation has been demonstrated in preparations from a wide variety of plant sources. This author reported that a high concentration of BSA3 in the grinding medium was necessary for cotton seedling particulate preparations to oxidize Krebs cycle intermediates (12). Because of this unusual requirement for BSA in the isolation technique, the study was extended to determine the influence of this material on oxidative phosphorylation. Certain other factors in the isolation and measurement technique were also considered.